Purification Solvent Management

Purification Solvent Management

Flexible and scalable modular pumps

Flexible and scalable modular pumps

As your lab’s productivity demands increase, so do your expectations for reliability, performance, and flexibility of your solvent delivery system. 

Waters innovative preparative pump technologies provide extraordinary performance and convenience for benchtop purification of microgram to multigram sample loads. The full range of Binary and Quaternary Gradient Modules have been designed to meet all your purification needs.

2545 Quaternary Gradient Module Purification Solvent Management

Overview

  • Easily configurable modules for a variety of purification protocols covering a wide range of sample matrices 
  • Exceptional flow rates provide you with the precision and accuracy you require 
  • Chromatographic reproducibility that you need to fully automate all your laboratory’s processes 
  • Exceptional solvent blending, automated solvent management, and pulse-free delivery, to meet even your most sensitive application challenges

Recommended Use: For flexible solvent delivery, whether for isocratic or gradient, or analytical or preparative.

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Features Header

2545 Binary Gradient Module

The 2545 Binary Gradient Module (BGM) is a high-pressure mixing binary gradient pump that serves as the primary solvent delivery device for the LC Prep AutoPurification System, enabling subsequent isolation and purification of targeted compounds.

Features:

  • Operates at analytical and preparative scales
  • Performs between flow rates of 0.50 to 150.00 mL/min with a pressure up to 6000 psi
  • Integrates solvent selection valves on each solvent delivery channel to allow for rapid solvent changeovers and system purges
  • Streamlines the purification process via MassLynx Software control using FractionLynx Application Manager

2535, 2545, and 2555 Quaternary Gradient Modules

The 2535, 2545, and 2555 Quaternary Gradient Modules (QGMs) are four solvent, low-pressure mixing gradient pumps that serve as solvent delivery devices for purification systems. Dual programmable flow paths enable the system volumes to change when the column size is changed.

2535 QGM

  • 50 mL/min capability for using columns up to 30 mm I.D. for purification of milligrams of material
  • High pressure operation at 6,000 psi (414 bar) to accommodate a wide variety of column lengths, diameters, and particle sizes

2545 QGM

  • 150 mL/min capability for using columns up to 50 mm I.D. for purification of milligrams to grams of material
  • High pressure operation at 6,000 psi (414 bar) up to 100 mL/min with a roll off to 5,000 psi (345 bar) at 150 mL/min to accommodate a wide variety of column lengths, diameters, and particle sizes

2555 QGM

  • 300 mL/min capability for using columns up to 75 mm I.D. for grams of material
  • High pressure operation of 3000 psi (207 bar) at 200 mL/min with a roll off to 2,500 psi (172 bar) at 300 mL/min to accommodate a wide variety of column lengths, diameters, and particle sizes

QGM Features:

  • Four-solvent gradient selection
  • Two fluidic pathways (small scale and large scale) to accommodate column selection and chromatographic efficiency
  • Control from MassLynx or Empower software or console, allowing scale up via a familiar software interface
  • Interfaces to a variety of detectors and peak fraction collectors for optimum purifications
  • Allows UV- or MS-directed purification with MassLynx Software
  • Leak detection is standard

1525EF HPLC Pump

The 1525EF HPLC Pump is a high-pressure mixing binary gradient pump that is utilized in many semi-preparative configurations. It provides you with precision and accuracy, delivering the chromatographic reproducibility your lab requires.

Features:

  • 225 μL pump heads deliver up to 22.5 mL/min
  • Optional FlexInject Manual Dual Injector Module for both analytical and semi-preparative injections
  • For semi-preparative applications: 10 to 30 mm inner diameter (I.D.) column separations
  • Maximum operating pressure: 6000 psi up to 22.5 mL/min

Prep Degasser

The standalone, two-channel Prep Degasser helps optimize laboratory uptime and productivity through Helium sparging, which eliminates the need to degas mobile phases. 

Features:

  • Can be used in combination with low-pressure mixing solvent delivery systems (2535 QGM and 1525EF pumps) for runs of 45 mL/min or less
  • Flow path materials and vacuum chambers are compatible with organic mobile phases vapors, for use with both reversed and normal phase chromatography

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HPLC Solvent Management encompasses a comprehensive array of processes and strategies dedicated to the efficient handling and regulation of solvents in high-performance liquid chromatography (HPLC) purification. This pivotal aspect of chromatography guarantees the utmost effectiveness and precision in the purification process. Key components of solvent management in HPLC purification include:

Solvent Selection: The critical choice of suitable solvents is essential for the success of HPLC purification. Solvents with compatible properties and appropriate purity levels are meticulously chosen based on sample characteristics and chromatographic conditions.

Solvent Mixing and Gradient Formation: Precision in mixing and blending solvents is indispensable for gradient elution in HPLC purification. This involves creating a gradient profile where the composition of the solvent mixture changes over time, facilitating the separation of target compounds from impurities.

Flow Rate Control: Precise control of solvent flow rates is crucial to achieving optimal chromatographic separation. Maintaining a consistent flow rate ensures the uniform elution of components, preserving the integrity of the separation process.

Column Compatibility: Ensuring compatibility between the solvent system and the HPLC column is paramount. Factors such as the stationary phase, particle size, and dimensions must be considered to achieve efficient and reproducible purification.

System Calibration: Regular calibration of the HPLC system is imperative to guarantee accurate solvent delivery. This involves verifying the precision of the solvent gradient, flow rates, and associated parameters, ensuring the reliability of the purification process.

Monitoring and Optimization: Continuous real-time monitoring of the solvent system during HPLC purification is vital. Parameters such as pressure, UV absorbance, and detector signals are assessed to optimize conditions, maximizing separation efficiency.

Waste Management: Proper disposal of waste solvents is integral to solvent management. Adhering to environmentally friendly practices and regulatory guidelines for solvent disposal is essential to maintain sustainability and compliance.

User Interface and Automation: Modern HPLC systems often incorporate user-friendly interfaces and automation features for solvent management. This streamlines the control of solvent parameters, facilitating setup, monitoring, and adjustment for operators.

Safety Protocols: Implementation of safety protocols is crucial to minimize risks associated with solvent handling. This includes proper labeling, storage, and handling procedures, along with the use of safety features in the HPLC system to prevent accidents.

Troubleshooting: A systematic approach to troubleshooting solvent-related issues is paramount. Identifying and promptly addressing concerns such as leaks, air bubbles, or fluctuations in solvent composition ensures the ongoing reliability of the purification process.

In summary, "HPLC Purification Solvent Management" involves a comprehensive range of practices and considerations, spanning from solvent selection to system calibration and safety protocols. Effective solvent management is instrumental in achieving the success of HPLC purification, optimizing conditions for the precise separation and collection of target compounds with high efficiency.

Purification Solvent Management FAQs

1. What is purification solvent management, and why is it important?
Purification solvent management refers to the processes and technologies used to efficiently regulate and handle solvents during liquid chromatography purification. It ensures precise solvent mixing, delivery, and flow rate control to optimize the separation and purification of target compounds. Proper solvent management is essential for maintaining chromatographic reproducibility, achieving high-resolution separations, and minimizing sample contamination. It also supports system reliability, reduces waste, and enhances safety.

Effective solvent management is critical for laboratories aiming to produce consistent and high-quality results, especially in applications requiring rigorous performance standards.

2. Can Waters solvent management systems handle both isocratic and gradient methods?
Yes, Waters solvent management systems are designed to handle both isocratic and gradient methods. Isocratic methods deliver a consistent solvent composition throughout the analysis, while gradient methods change the solvent composition over time to improve the separation of complex mixtures. Waters systems, such as the 2545 Binary Gradient Module and 2535, 2545, and 2555 Quaternary Gradient Modules, provide exceptional flexibility for both approaches.

3. How do I choose the right pump for my purification needs?
Selecting the right pump depends on your sample size, throughput requirements, and column specifications. For smaller-scale purifications, pumps like the 1525EF HPLC Pump, with flow rates up to 22.5 mL/min, are ideal. For larger sample loads, the 2545 Binary Gradient Module or 2535, 2545, and 2555 Quaternary Gradient Modules offer flow rates up to 300 mL/min, supporting columns up to 75 mm I.D.

Consider whether you need high-pressure mixing for precise gradients, dual fluidic pathways for scalability, or automated solvent management for improved efficiency. Matching the pump's capabilities to your application ensures optimal performance.

4. What types of samples and matrices are compatible with Waters purification systems?
Waters purification systems are compatible with a wide range of samples and matrices, including small molecules, biomolecules, pharmaceuticals, natural products, environmental samples, and industrial chemicals. These systems excel in handling complex sample matrices, such as plasma, urine, soil, and plant extracts, and are compatible with reversed-phase, normal-phase, ion exchange, and size-exclusion chromatography.

The flexibility of Waters systems allows for efficient purification of analytes across different chemistries, ensuring high recovery and resolution regardless of the sample's complexity or composition.