ACQUITY UPLC Protein BEH SEC Column, 200Å, 1.7 µm, 4.6 mm X 150 mm, 10K - 500K, 1/pk

An integral part of Waters’ ACQUITY UPLC SEC System solution, the ACQUITY UPLC Protein BEH SEC columns are designed to characterize proteins ranging in molecular weight from 10,000 to 450,000 Daltons, including monoclonal IgG monomers from aggregates.

Achieve quick and accurate aggregate determinations up to 10 times faster than other conventional HPLC-based SEC methods when using the ACQUITY UPLC Protein BEH SEC Column, 125Å, 1.7 µm, 4.6 mm x 150 mm, 1/pk and BEH125 SEC Protein Standard Mix, whether the pore size is 200Å, 125Å, or something else.

Developed to be used on HPLC instrumentation and as a complement to existing lines of smaller particle sizes, UPLC based SEC columns for SEC, the ACQUITY UPLC Protein BEH SEC column delivers a longer SEC column lifespan with less non-desired protein/column interactions than silica-based SEC columns. Easily develop and/or transfer SEC methods based on instrumentation, required protein component resolution, and sample throughput requirements with the innovation of ACQUITY UPLC Protein BEH SEC columns.

Guarantee unprecedented column consistency and an increased confidence in validated methods due to the comprehensive testing that all ACQUITY UPLC Protein BEH SEC columns undergo. All particles are synthesized in ISO-certified manufacturing facilities that feature state-of-the-art technology and use high-quality raw materials. Extensive quality control testing is performed throughout the QC synthetic process. Each batch of columns is also tested with relevant proteins in order to ensure consistency across batches and instill confidence in tried and true methods.

Provide a stable chemistry with minimal non-desired secondary interactions for proteins and peptides due to the SEC chemistries that are used in these columns, as they are based on Waters’ Ethylene Bridged Hybrid (BEH) particle technology and diol-bonded surface. This combination results in improved column stability, performance, and a lifespan that is unprecedented in traditional chromatographic columns. These benefits are well established for use by scientists that work with varying biological compounds with stability and performance attributes not found in most 100% silica-based particles.

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FAQs About ACQUITY UPLC Protein BEH SEC Column, 200 Å, 1.7 µm, 4.6 mm X 150 mm, 1/pk

1. What is the particle and pore size of the ACQUITY UPLC Protein BEH SEC Column?
The ACQUITY UPLC Protein BEH SEC Column, 200 Å, 1.7 µm, 4.6 mm X 150 mm, 1/pk features a particle size of 1.7 µm. This small particle size enables high-efficiency separations and faster analysis times compared to larger particle size columns.
The pore size of 200 Å is optimized for the separation of proteins. This pore size allows for the effective separation of proteins based on their molecular weight while minimizing non-specific interactions that can lead to peak broadening and reduced resolution.

2. What is the typical retention time range for proteins in this column?
The retention time range for proteins on the ACQUITY UPLC Protein BEH SEC Column can vary depending on factors such as protein size, mobile phase composition, and flow rate. However, typically, proteins with molecular weights ranging from approximately 10 kDa to 1 MDa can be separated in this column.
Smaller proteins will elute earlier in the chromatogram, while larger proteins will elute later. By adjusting the mobile phase composition and flow rate, the retention time range can be further optimized to suit specific separation needs.

3. What is the typical flow rate and pressure range for this column?
The typical flow rate range for the ACQUITY UPLC Protein BEH SEC Column is 0.3-1.0 mL/min. Higher flow rates can be used to reduce analysis time but may result in lower resolution. Lower flow rates can improve resolution but increase analysis time.
The pressure range for this column is typically between 500-2000 psi. The actual pressure will depend on the specific flow rate and mobile phase viscosity. It's important to ensure that the system pressure does not exceed the maximum operating pressure of the instrument and column to avoid damage.