BioResolve SEC Column 4.6x150 mm mAb Size Variant Standard 176004592

SKU: 176004592
BioResolve SEC mAb Column, 200Å, 2.5 µm, 4.6 x 150 mm, 1/pk with mAb Size Variant Standard

BioResolve SEC mAb Column | 176004592


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Product Description

The BioResolve SEC mAb 4.6 x 150 mm Column contains 2.5 µm BEH particle technology and is application specifically tested with the mAb Size Variant Standard to ensure accurate and highly reproducible quantitation of monoclonal antibody (mAb) monomers from frequently associated high molecular weight aggregates (≥300,000 Da) and lower molecular weight fragments (e.g., ≤100,000 Da).  This column is also available with Water mAb Size Variant Standard (PN 186009429) at no extra charge.

Specifications

  • Chemistry

    SEC

  • Separation Mode

    SEC

  • Particle Substrate

    Hybrid

  • pH Range Min

    2.5 pH

  • pH Range Max

    8 pH

  • Temperature Limits

    60 C

  • Maximum Pressure

    4500 psi (310 Bar)

  • Endcapped

    No

  • Bonding Technology

    Diol (OH)

  • Silanol Activity

    Low

  • Molecular Weight Range Min

    10000

  • Molecular Weight Range Max

    450000

  • Particle Shape

    Spherical

  • Particle Size

    2.5 µm

  • Endfitting Type

    Parker-style

  • Pore Size

    200 Å

  • QC Tested

    Protein

  • Format

    Online Column

  • Packing Solvent

    10% acetonitrile/90% 25 mM sodium phosphate pH 7.0 + 100 mM KCl

  • Surface Area

    220

  • System

    HPLC

  • Particle Technology

    BEH

  • Technique

    LC

  • USP Classification

    L33

  • Inner Diameter

    4.6 mm

  • Length

    150 mm

  • Carbon Load

    12 %

  • eCord

    Yes

  • UNSPSC

    41115711

  • Application

    Protein

  • Brand

    BioResolve

  • Product Type

    Columns

  • Units per Package

    1 pk

Product Support

Documents

Documents



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BioResolve SEC mAb Column, 200Å, 2.5 µm, 4.6 x 150 mm, 1/pk with mAb Size Variant Standard

Don’t just change the way you quantitate monoclonal antibodies, transform them for the better with the help of the BioResolve SEC mAb Column. This column is specifically designed for the accurate, reproducible quantitation of monoclonal antibody monomers from both high and lower molecular weight fragments. But to ensure you only receive the most accurate and best-performing column time and again, each BioResolve SEC mAb Column is QC tested with the Waters Size Variant Standard. We’ve included this QC reference material free of charge with your column so you can benchmark your own LC-MS systems properly. Upgrade your mAb separations with the BioResolve SEC mAb Column now.

A major concern when hard at work in the lab on monoclonal antibody quantitation is column contamination. Although proper sample preparation processes should be followed to ensure you only have the cleanest samples running through your analytical column, there’s always a chance that very small contaminants in the mobile phase stream can enter and foul your column over time. To increase overall column lifetimes and prevent that contamination, we’re happy to provide the BioResolve SEC mAb Guard. This guard column seamlessly incorporates into your existing workflow to provide enhanced protection against contaminants. Plus, when the guard has become worn and needs to be replaced, you can do so with another genuine replacement from Waters.

At the heart of the BioResolve SEC mAb Column is Ethylene-Bridged Hybrid (BEH) particle technology. Thanks to the intrinsic chemical stability of the BEH particle, you can develop methods with a wide pH range with no concerns about performance. And because we care deeply about performance, this column can be used on UHPLC, HPLC and UPLC systems. This especially comes in handy when developing methods.

Stock up your lab today with all of the incredible instruments, products and resources from Waters, and you’ll be well on your way to changing your experiments for the better. We have everything from quality control reference materials to lab informatics, making us a one-stop shop for all your LC-MS needs. Shop lab equipment today.

What’s the BioResolve SEC mAb Column’s Molecular Weight Range?

The BioResolve SEC mAb Column offers a molecular weight range of 10,000 to 450,000.