Chemistry |
SEC |
Separation Mode |
SEC |
Particle Substrate |
Hybrid |
pH Range Min |
2.5 pH |
pH Range Max |
8 pH |
Temperature Limits |
60 C |
Maximum Pressure |
4500 psi (310 Bar) |
Endcapped |
No |
Bonding Technology |
Diol (OH) |
Silanol Activity |
Low |
Molecular Weight Range Min |
10000 |
Molecular Weight Range Max |
450000 |
Particle Shape |
Spherical |
Particle Size |
2.5 µm |
Endfitting Type |
Parker-style |
Pore Size |
200 Å |
QC Tested |
Protein |
Format |
Online Column |
Packing Solvent |
10% acetonitrile/90% 25 mM sodium phosphate pH 7.0 + 100 mM KCl |
Surface Area |
220 |
System |
HPLC |
Particle Technology |
BEH |
Technique |
LC |
USP Classification |
L33 |
Inner Diameter |
4.6 mm |
Length |
150 mm |
Carbon Load |
12 % |
eCord |
Yes |
UNSPSC |
41115711 |
Application |
Protein |
Brand |
BioResolve |
Product Type |
Columns |
Units per Package |
1 pk |
BioResolve SEC mAb Column, 200Å, 2.5 µm, 4.6 x 150 mm, 1/pk with mAb Size Variant Standard
Don’t just change the way you quantitate monoclonal antibodies, transform them for the better with the help of the BioResolve SEC mAb Column. This column is specifically designed for the accurate, reproducible quantitation of monoclonal antibody monomers from both high and lower molecular weight fragments. But to ensure you only receive the most accurate and best-performing column time and again, each BioResolve SEC mAb Column is QC tested with the Waters Size Variant Standard. We’ve included this QC reference material free of charge with your column so you can benchmark your own LC-MS systems properly. Upgrade your mAb separations with the BioResolve SEC mAb Column now.
A major concern when hard at work in the lab on monoclonal antibody quantitation is column contamination. Although proper sample preparation processes should be followed to ensure you only have the cleanest samples running through your analytical column, there’s always a chance that very small contaminants in the mobile phase stream can enter and foul your column over time. To increase overall column lifetimes and prevent that contamination, we’re happy to provide the BioResolve SEC mAb Guard. This guard column seamlessly incorporates into your existing workflow to provide enhanced protection against contaminants. Plus, when the guard has become worn and needs to be replaced, you can do so with another genuine replacement from Waters.
At the heart of the BioResolve SEC mAb Column is Ethylene-Bridged Hybrid (BEH) particle technology. Thanks to the intrinsic chemical stability of the BEH particle, you can develop methods with a wide pH range with no concerns about performance. And because we care deeply about performance, this column can be used on UHPLC, HPLC and UPLC systems. This especially comes in handy when developing methods.
Stock up your lab today with all of the incredible instruments, products and resources from Waters, and you’ll be well on your way to changing your experiments for the better. We have everything from quality control reference materials to lab informatics, making us a one-stop shop for all your LC-MS needs. Shop lab equipment today.
What’s the BioResolve SEC mAb Column’s Molecular Weight Range?
The BioResolve SEC mAb Column offers a molecular weight range of 10,000 to 450,000.